Pristine Sample Laboratory
From the air shower we enter the Pristine Sample Laboratory Laboratory.
It is important to note that the Lunar Sample Laboratory Facility is a working laboratory, not a museum.
In this room, pristine lunar samples for research, for education, and for display are prepared for
distribution to recipients located at 85 universities and institutions throughout the world. We
presently have international customers in Australia, Canada, England, France, Germany, Hungary, India,
Ireland, Japan, and Switzerland. Hundreds of scientists, professors, and students from all over the
world attend the annual Lunar and Planetary Science Conference at which new lunar and planetary
research findings are discussed.
"Pristine" samples, or samples which have not been out of the laboratory since they were returned from
the Moon, are processed in specially manufactured stainless steel cabinets filled with flowing nitrogen
gas which has been carefully purified to remove water and oxygen. Before the samples were collected,
they had been in a vacuum on or near the lunar surface for millions to billions of years. If the
samples were exposed to the Earth's humid atmosphere, they would react with it. Small grains of iron
in the lunar samples would combine with oxygen to form rust; glasses and minerals would combine with
the water in air to form clays. Nitrogen gas is used in the cabinets because it provides an environment
with minimum reaction to the samples.

Rubber gloves made of Neoprene, which are fitted to holes in the sides of the cabinets, let workers
reach the samples inside the air-tight, sealed cabinets. The nitrogen gas pressure is slightly
greater than the air pressure. Should a leak develop, nitrogen would rush out from the cabinet and
prevent the air from entering and contaminating the samples. Since a serious leak could flood the
laboratory with nitrogen gas, the oxygen level in the room air is carefully monitored for personnel
safety. Because oxygen and water in air slowly permeate the rubber gloves and enter the cabinets,
the nitrogen is kept flowing in order to prevent concentrations of these reactive gases from building
up inside the cabinets. Automatic hourly testing of each cabinet assures that the water and oxygen
concentrations are maintained at less than 20 molecules of oxygen and 50 molecules of water for every
one million molecules of nitrogen.

After the earlier Apollo missions, NASA was more concerned about how the samples might contaminate the
Earth than about how the samples would be contaminated by the Earth. To avoid cross-contamination of
samples from different places on the Moon, samples from different missions are not processed together
in the same cabinet, but one or more cabinets are designated for processing samples from a particular
mission. When cabinets become dusty from extensive processing or are needed for processing samples
from a different mission, they are cleaned using ultra-pure water.

The nitrogen cabinets are equipped with tools, containers, and other pieces equipment which have been
specially cleaned and packaged in hermetically sealed bags. The materials which are deemed acceptable
for the manufacturing of these items are carefully considered. The only materials which are allowed to
touch the samples are aluminum, stainless steel and Teflon. Teflon gloves are worn over rubber gloves
used for picking up rocks. Samples are packaged in Teflon bags, plastic vials, aluminum or stainless
steel containers. Two hermetically sealed Teflon bags are placed on the outside of all containers with
samples slated for storage or distribution to a Principal Investigator.
All sample processing is carefully documented in folders called data packs. Pieces which have been
removed from the original sample are assigned new sample numbers, and the masses for all samples are
accounted for to the nearest 10 milligrams. Photographs are taken of the samples before and after
subdivision, and the orientations of new pieces relative to the original sample are recorded as
accurately as possible.